resources
CRISPR Cas9 Detailed Protocol
From High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome
Jorgensen lab plasmids
are available from
iBiology Talk by Erik Jorgensen
SapTrap builder
SapTrap builder is a program for guided construction of reagents for CRISPR-based genome engineering.
ApE
ApE is an integrated program for plasmid construction and visualization. It includes a sequence editor, a linear text map identifying sequence features such as restriction sites and ORFs, and a graphic view that highlights sites and features in your sequences. A program designed by a molecular biologist, for molecular biologists.
Wormbuilder
Methods and reagents for using Mos1 to manipulate the C. elegans genome
Moving Worms
A moving chapter from Erik’s CSHL Course